Real-time Detection of Trace Copper in Brain and Kidney of Fish for Medical Diagnosis
نویسندگان
چکیده
For the detection of trace copper to be used in medical diagnosis, a sensitive handmade carbon nanotube paste electrode (PE) was developed using voltammetry. Analytical optimized conditions were found at 0.05 V anodic peak current. In the same conditions, various common electrodes were compared using stripping voltammetry, and the PE was found to be more sharply sensitive than other common electrodes. At optimum conditions, the working ranges of 3~19 μgL(-1) were obtained. The relative standard deviation of 70.0 μgL(-1) was determined to be 0.117% (n = 15), and the detection limit (S/N) was found to be 0.6 μgL(-1) (9.4 × 10(-9) M). The results were applied in detecting copper traces in the kidney and the brain cells of fish.
منابع مشابه
Bioaccumulation and Depuration of Copper in the Kidney and Liver of a Freshwater Fish, Capoeta fusca
Background: This study aims to investigate the patterns of bioaccumulation and depuration of copper in the selected kidney and liver of Capoeta fusca. Methods: The fish were collected between September and November 2010 from a qanat in Birjand. They were exposed to two types treatments with copper (0.25 and 0.75 mg/L) for a period of 41 days. The fish under study were exposed to the above-ment...
متن کاملHistopathological effects of acute copper sulphate exposure on kidney, liver and gill of common carp, Cyprinus carpio, fingerlings
In the present study histological effects of copper sulphate on, 180 common carp, Cyprinus carpio, fingerlings were studied. In this regard, fish of treatment groups were exposed to 2, 4, 6, 8 and 10 ppm of copper sulphate during 4 days. Behaviours and clinical signs of fish were recorded during the experiment, and samples were done from kidney, liver and gill of moribund and fresh dead fish, a...
متن کاملEvaluation of Microscopy Sensitivity, Specificity in Detection of P. falciparum and P. vivax, Using Monoplex real-time PCR, Gezira, Sudan
Background: Malaria is still account for 200 million cases annually. Microscopy is the gold standard technique for malaria parasites detection. PCR-based techniques can detect malaria infections with high sensitivity. The study aimed to evaluate the sensitivity of microscopy technique in the detection of P. falciparum and P. vivax, using monoplex real-time PCR, Gezira State, Central Sudan. Met...
متن کاملDevelopment of ultrasound-assisted emulsification microextraction for the determination of trace zinc and copper by flame atomic absorption spectrometry
In this work, we developed a method based on ultrasound-assisted emulsification microextraction (USAEME) for the determination of zinc and copper by flame atomic absorption spectrometry (FAAS). The method is based on the use of the organic solvent carbon tetrachloride (CCl4) as an extraction solvent. In order to obtain a high enrichment factor, the effect of different parameters affecting the c...
متن کاملComparison of classical cytogenetics versus interphase FISH in diagnosis of mosaic form of Turner syndrome
Abstract Background: Mosaic form of turner syndrome that represented by two or more cell lines in an affected individual, often has limitation for detection with classical cytogenetic methods. The present study was carried out to compare the efficiency of interphase Fluorescence In Situ Hybridisation (FISH) and cytogenetic techniques in detection of mosaic form of turner syndrome. Method...
متن کامل